Consulta: subjectFacets:"Ovalbumin"
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Data/hora: 16/05/2024 09:51:57

A model of chronic IgE-mediated food allergy in ovalbumin-sensitized mice
Provedor de dados: 56
Autores: Saldanha,J.C.S.; Gargiulo,D.L.; Silva,S.S.; Carmo-Pinto,F.H.; Andrade,M.C.; Alvarez-Leite,J.I.; Teixeira,M.M.; Cara,D.C.
Food allergy is most frequently the result of IgE-mediated hypersensitivity reactions. Here, we describe a chronic model in which some of the intestinal and systemic consequences of continuous egg white solution ingestion by ovalbumin-sensitized eight-week-old BALB/c mice, 6 animals per group, of both sexes, were investigated. There was a 20% loss of body weight that began one week after antigen exposure and persisted throughout the experiment (3 weeks). The sensitization procedure induced the production of anti-ovalbumin IgG1 and IgE, which were enhanced by oral antigen exposure (129% for IgG1 and 164% for IgE, compared to sensitization values). Intestinal changes were determined by jejunum edema at 6 h (45% Evans blue extravasation) and by a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food allergy; Ovalbumin; Murine model; IgE; Hypersensitivity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600005

Albumen protein and functional properties of gelation and foaming
Provedor de dados: 63
Autores: Alleoni,Ana Cláudia Carraro.
Hen eggwhite proteins have been extensively utilized as ingredients in food processing because of their unique functional properties, such as gelling and foaming. This work reviews the molecular basis of the eggwhite proteins targeting the development of these functional properties during processing.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovalbumin; Coagulation; Gel; Denaturation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000300013

Coupling of palmitate to ovalbumin inhibits the induction of oral tolerance
Provedor de dados: 56
Autores: Oliveira,F.M.; Silva-Neto,A.F.; Silva,C.A.; Gontijo,C.M.
Oral tolerance is a phenomenon that may occur in animals exposed to protein antigens for the first time by the oral route. They become unable to produce immune responses at the levels normally observed when they are immunized parenterally with antigen in the presence of adjuvants. Lipids have been used as adjuvants for both parenteral and oral immunization. In the present study we coupled ovalbumin with palmitate residues by incubating the protein with the N-hydroxysuccinimide palmitate ester and tested the preparation for its ability to induce oral tolerance. This was performed by giving 20 mg of antigen to mice by the oral route 7 days prior to parenteral immunization in the presence of Al(OH)3. Mice were bled one week after receiving a booster that was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Oral tolerance; Lipid-protein conjugates; Antigen absorption; Gut mucosa; Ovalbumin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100009

Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates
Provedor de dados: 52
Autores: Oliveira,Flávia Márcia; Ruiz-de-Souza,Valéria; Campana-Pereira,Maria Aparecida; Gontijo,Cristiano Machado.
One of the mechanisms by which adjuvants are believed to promote T-cell activation and prevent induction of oral tolerance is by up-regulating the expression of co-stimulatory molecules on antigen presenting cells. Mice treated orally with palmitoyl-ovalbumin conjugates become immunized, while those treated with native ovalbumin (Ova) become tolerant. Cells from the peritoneal cavity of B6D2F1 mice were cultured in the presence of 0.01, or 0.1 mg/100ml of either Ova, or palmitoyl-Ova and tested for the presence of cell markers. PE-conjugated anti-mouse CD80, CD86, and CD11b antibodies as well as biotin-PE were used to stain the antigen-activated peritoneal cells. A significant increase in the expression of CD86 and CD80 was observed following in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovalbumin; Palmitic Acid; Cellular Immunity; CD86; CD80; CD11b.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600011

Gene Polymorphisms are Associated with Eggshell Ultrastructure Organization in Hens
Provedor de dados: 89
Autores: Wang,D; Liao,B; Zhang,Q; Liu,JS; Duan,Z; Hou,Z; Ning,Z.
ABSTRACT  Background:  Eggshell ultrastructure organization, including effective layer thickness, mammillary layer thickness, and average size of mammillary cones, is important for breeding and significantly influences eggshell mechanical properties. Several matrix proteins were known to be important in eggshell formation. However, the proteins and variations that determine eggshell ultrastructure organization are not known.  Results:  In this study, 17 single-nucleotide polymorphisms of three major genes in a hen population using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Five single-nucleotide polymorphisms with a very low minor allele frequency (&lt; 1%) were excluded from further analysis. The remaining 12...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calmodulin1; Eggshell ultrastructure organization; Ovalbumin; Ovocleidin-116.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100129

Indirect effects of oral tolerance to ovalbumin interfere with the immune responses triggered by Schistosoma mansoni eggs
Provedor de dados: 56
Autores: Carvalho,C.R.; Lenzi,H.L.; Correa-Oliveira,R.; Vaz,N.M.
The objective of the present study was to investigate whether the injection of a tolerated protein (indirect effects) affects the formation of granulomas around Schistosoma mansoni eggs trapped in the lungs after intravenous (iv) injection into normal (noninfected) C57BL/6 mice (6 animals per group). To induce oral tolerance to chicken egg ovalbumin a 1/5 dilution of egg white in water was offered ad libitum in a drinking bottle for 3 days. Control mice received water. After 7 days, control and experimental animals were injected iv with 2,000 S. mansoni eggs through a tail vein. In some mice of both groups the iv injection of eggs was immediately followed by intraperitoneal (ip) immunization with 10 µg of dinitrophenylated conjugates of ovalbumin (DNP-Ova)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Oral tolerance; Ovalbumin; Granuloma; Schistosoma mansoni.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000012

Interruption of recently induced immune responses by oral administration of antigen
Provedor de dados: 56
Autores: Conde,A.A.; Stransky,B.; Faria,A.M.C.; Vaz,N.M.
Interest in oral tolerance has been renewed in the last few years as a possibility of intervention in human autoimmune diseases. An obstacle in this direction is that, although easily induced in animals virgin of contact with the antigen, oral tolerance becomes hard to induce in previously immunized animals. The present results show that there is an early period after primary immunization in which prolonged oral exposure to the antigen may arrest ongoing immune responses. Beyond this period, oral exposures to the antigen become ineffective and may actually boost immune responses. The end of the susceptible period coincides with the emergence of free specific antibodies in serum. However, the previous administration of purified anti-ovalbumin antibodies (40...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Suppression; Antibody response; Oral tolerance; Ovalbumin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300008

Oral tolerance induction with altered forms of ovalbumin
Provedor de dados: 56
Autores: Stransky,B.; Faria,A.M.C.; Vaz,N.M.
As a T cell-dependent phenomenon, oral tolerance is not expected to depend necessarily on native configuration of antigens. We investigated the induction of oral tolerance with modified ovalbumin (Ova). Oral administration of heat-denatured (HD-Ova) and cyanogen bromide-degraded ovalbumin was less effective than native Ova in inducing oral tolerance in B6D2F1 mice. HD-Ova was effective in suppressing delayed-type hypersensitivity (DTH) reactions but did not suppress specific antibody formation. Injection of Ova directly into the stomach, but not into the ileum or cecum, suppressed subsequent immunization to DTH reactions. Gavage with protease inhibitors (aprotinin or ovomucoid) before gavage with Ova was ineffective in blocking tolerance induction....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigen; Digestion; Oral tolerance; Mouse; Ovalbumin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300009

Sildenafil decreases rat tracheal hyperresponsiveness to carbachol and changes canonical transient receptor potential gene expression after antigen challenge
Provedor de dados: 56
Autores: Sousa,C.T.; Brito,T.S.; Lima,F.J.B.; Siqueira,R.J.B.; Magalhães,P.J.C.; Lima,A.A.M.; Santos,A.A.; Havt,A.
Inhibition of type-5 phosphodiesterase by sildenafil decreases capacitative Ca2+ entry mediated by transient receptor potential proteins (TRPs) in the pulmonary artery. These families of channels, especially the canonical TRP (TRPC) subfamily, may be involved in the development of bronchial hyperresponsiveness, a hallmark of asthma. In the present study, we evaluated i) the effects of sildenafil on tracheal rings of rats subjected to antigen challenge, ii) whether the extent of TRPC gene expression may be modified by antigen challenge, and iii) whether inhibition of type-5 phosphodiesterase (PDE5) may alter TRPC gene expression after antigen challenge. Sildenafil (0.1 µM to 0.6 mM) fully relaxed carbachol-induced contractions in isolated tracheal rings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Ovalbumin; Capacitative Ca2+ entry; Transient receptor potential proteins.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600010

The effects of ovalbumin as a protein source during the in vitro production of bovine embryos
Provedor de dados: 96
Autores: Tetzner,Tatiane Almeida Drummond; Saraiva,Naiara Zoccal; Perecin,Felipe; Niciura,Simone Cristina Méo; Ferreira,Christina Ramires; Oliveira,Clara Slade; Garcia,Joaquim Mansano.
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine embryo; In vitro culture; Ovalbumin; Protein source.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001000010